Tuesday, 05. November 2019, Francis Crick Institute, Practical Flow Cytometry Course

from 05. November 2019 - 9:00
till 07. November 2019 - 17:00

Francis Crick Institute

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Event description
This three-day course will serve as an introduction to the use of flow cytometry to less experienced delegates. We will cover the basics of fluorescence and the flow cytometer and demonstrate some of the more commonly used applications to illustrate how the cytometer can be used. We will cover multicolour fluorescence and compensation, DNA and cell cycle kinetics, cell death and apoptosis and receptor quantification. Delegates will have the opportunities to gain hands-on experience using either a BDLSRII or an LSR Fortessa and data generated will also be used to help illustrate how best to perform data analysis.
Places will be limited to 12 delegates to ensure maximum interaction. The lead tutor will be Derek Davies.
For further information, contact: stp-training@
Agenda (subject to change) Day One: Lectures -Fluorescence and Fluorochromes, how a cytometer works. Practicals – Instrument set-up, compensation using beads
Day Two: Lectures: DNA and cell cycle analysis,  Practicals - Compensation experiment, Viability dyes and DNA analysis, data analysis
Day Three: Lectures; Apoptosis, titration and cell proliferation. Practicals: Cell death, antibody titration, dye dilution for proliferation.

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